In vitro RNA-mediated gene silencing of Fusarium oxysporum f.sp. cubense from Ecuador and assessment of RNAi molecule stability in banana plants

Table 1 Primers F.r obtaining double-stranded RNA F.r Fusarium oxysporum F. Sp. Cubense race 1 and RT-qPCR reaction

Target gene		Secuence	Size of fragment
Beta tubulin (Focβ-tub)- for dsRNA	forward 5ˈ- 3ˈ	GCGTAATACGACTCACTATAGGGAGACTTGAGCCTGGTACCATGGA
Beta tubulin (Focβ-tub)- for dsRNA	Reverse 5ˈ- 3ˈ	GCGTAATACGACTCACTATAGGGAGAGGAGCAAAGCCAACCATGAA	614 bp
C5 - Sterol desaturase (FocERG3) - for dsRNA	forward 5ˈ- 3ˈ	GCGTAATACGACTCACTATAGGGAGATGGATCTTTGGGCTTCTCGT
C5 - Sterol desaturase (FocERG3) - for dsRNA	Reverse 5ˈ- 3ˈ	GCGTAATACGACTCACTATAGGGAGAATCGGCCCTCTGACATCTTC	622 bp
Chitin synthase 1 (FocChs1) - for dsRNA	forward 5ˈ- 3ˈ	GCGTAATACGACTCACTATAGGGAGAGGACAAGCCTCTCGAGTCTT
Chitin synthase 1 (FocChs1) - for dsRNA	Reverse 5ˈ- 3ˈ	GCGTAATACGACTCACTATAGGGAGAGCATGACGAAGGCCAAGTAG	620 bp
Adenylate cyclase (FocAdcy) - for dsRNA	forward 5ˈ- 3ˈ	GCGTAATACGACTCACTATAGGGAGATGCACGAAAATTTTGGTCACATATTCGC
Adenylate cyclase (FocAdcy) - for dsRNA	Reverse 5ˈ- 3ˈ	GCGTAATACGACTCACTATAGGGAGACCATAATTTTGCCCGAGGCGC	660 bp
Chitin synthase 1 (FocChs1)– for RT-qPCR	forward 5ˈ- 3ˈ	GAATGCTGTCCAGCCTCAGT	127 bp
Chitin synthase 1 (FocChs1)– for RT-qPCR	Reverse 5ˈ- 3ˈ	AGGTCGCAAATCGTAACCGT	127 bp
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)– for RT- qPCR [29]	forward 5ˈ- 3ˈ	CGTCGATCTTACTGTCCGCC	110 bp
	Reverse 5ˈ- 3ˈ	CCTCAGTGTAGGCCAGAACG	110 bp

ISSN: 1756-0500