Fig. 1
Spore inhibition of Fusarium oxysporum f. sp. cubense race 1 with dsRNA. (a) Inhibition with dsRNA at 1000 mg/L on isolate EC35-G-GM1 of FocR1 with dsRNA-FocChs1, dsRNA-FocB-tub, dsRNA-FocERG3 and dsRNA-FocAdcy. (b) Bar graph of spore inhibition with dsRNA-FocChs1 (100%), dsRNA-FocB-tub (75.87%), dsRNA-FocERG3 (80.18%) and dsRNA-FocAdcy (99.37%) at concentrations of 1500 mg/L on spores of isolate EC35-G-GM1 of FocR1. Bars represent standard errors. Equal letters above bars are not significantly different at p > 0.05. (c) Box plot of the mean inhibitory concentration (IC50) in mg/L with dsRNA-FocChs1, dsRNA-FocAdcy, dsRNA-FocB-tub and dsRNA-FocERG3, on isolate EC35-G-GM1 of FocR1. 9 observations were included for each gene. (d) Expression level of FocChs1 gene following Foc spore growth with dsRNA-FocChs1 at 24 and 48 h (hrs). The expression level of FocChs1 was normalized to the expression of Foc GAPDH. Expression level was performed by RT-qPCR in three technical replicates and three biological replicates independently. Statistical significance (Student’s t-test) between the treatment and the control: *, P < 0.05