Fig. 3
From: Thermal potentiation improves IFN-γ production but not cytotoxicity in human CAR-T cells
Exposure to febrile temperature does not improve the cell killing ability of STEAP1 CAR-T cells. (A) Representative images illustrating a FACS workflow for the detection of caspase 3 positive 22RV1 target cancer cells. The T cells, selected and activated as above, were co-cultured with 22RV1 target cancer cells, after which the induction of caspase 3 in target cells was evaluated. (B) The corresponding quantification of caspase 3 positive target cells. Statistical analysis was performed using unpaired T test. Colors indicate individual donors as in A. (C) Change in the levels of indicated transcripts in human T cells, detected by RT-qPCR. The cells were harvested before co-culturing with cancer cells (see Fig. 1A)