Fig. 1
From: Thermal potentiation improves IFN-γ production but not cytotoxicity in human CAR-T cells
Protocol used to assess the effect of febrile temperature on human T cells. (A) The non-transduced T cells (NT-T) or CAR-T cells were activated for 24 h with antibodies against CD3 and CD28 at either 37 oC or 39.5 oC. Next, the T cells were co-cultured with STEAP1 + target cancer cells (22RV1) for 24 h at 37 oC. Then, IFN-γ and caspase 3 positive cells were detected by FACS. (B) The numbers of CAR-T cells were determined (by trypan blue staining) after the activation for 24 h at 37 oC or 39.5 oC. Note that temperature had no impact on cell numbers, implying no change in cell proliferation