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Fig. 3 | BMC Research Notes

Fig. 3

From: An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation

Fig. 3

Transient expression of pLCS vectors in protoplasts and pJRA vectors in tobacco. Confocal microscopy of Arabidopsis protoplasts transformed with pLCS vectors and Nicotiana benthamiana leaves transformed using the pJRA vectors. A-B Images of various genes tagged with basic (constitutively fluorescent) tags in protoplasts (A) and tobacco (B). Individual channels are shown in Figure S1 & S2. C Close up images of 3D projected ARF19 condensates tagged with Dendra2 and moxMaple3 before (pre) and after (post) photoconversion by a 405 nm laser. D Images of NBR1 tagged with Dendra2 and moxMaple3. The yellow channel represents the green form and magenta the red form. E Ratiometric images of ARF19 tagged with the mScarlet-I-mNeonGreen (mSc-I-mNG) tandem fluorescent timer in protoplasts. As mNeonGreen has a faster maturation than mScarlet-I, the ratio mSc-I / mNG reflects the age of the protein (i.e., a larger ratio equates to older protein). Scale bar 25 μm. F Ratiometric images of NLP7 tagged with the mScarlet-I-mNeonGreen tandem fluorescent timer in tobacco leaves

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BMC Research Notes

ISSN: 1756-0500

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