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Fig. 2 | BMC Research Notes

Fig. 2

From: An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation

Fig. 2

pJRA Series Binary Vectors. pJRA-GW was created from pMCS-GW [1] by replacing the kanamycin resistance gene (KanR) with the spectinomycin resistance gene (SpecR). The pJRA vectors are binary vectors designed for agrobacterium-mediated transformation and improve upon the previously published pMCS-GW and pUBQ10-YFP-GW vectors. Between the left and right borders (LB and RB) the BASTA resistance gene (BAR) is flanked by the manopine synthase promoter and manopine synthase terminator providing resistance to BASTA in transformed plants. As in the pLCS series, the pUBQ10 drives the expression of the fluorophore in frame with the gateway cassette

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BMC Research Notes

ISSN: 1756-0500

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