Fig. 3

Cell localization of ΔE3-10 Sdha protein and prediction of its 3D structure. A. Confocal images obtained in fixed U2OS cells co-transfected with the mitochondrial targeting signal of the cytochrome c oxidase subunit 8A (COXA) fused to a mCherry protein as a mitochondria marker (mitoCherry) and constructs containing the Sdha isoforms fused to an EGFP protein. Green, EGFP; Red, mCherry; Blue, nucleus. Individual and merge images are shown. B Prediction of the 3D structure of the canonical and ΔE3-10 Sdha proteins using the SWISS model server. The FAD-binding domain, the capping domain, the helical domain, and the C-terminal domain are depicted in different colors. The Δ3-10 Sdha isoform contains the helical domain and the C-terminal domain