Table 1 The thermal cycling program specifications used in real-time PCR
From: Antiviral activity of silver nanoparticles against H1N1 influenza virus
Cycle count | Time | Temperature (°C) | Real-time PCR steps |
|---|---|---|---|
1 | 30 min | 50 | cDNA synthesis |
2 min | 95 | Initial denaturation | |
45 | 15 s | 95 | Denaturation |
30 s | 58 | Primer annealing | |
30 s | 72 | Extension |