Fig. 7

Immunoblot and expression analysis of the Akt, mTOR, and PI3K-Ca of MCF-7 cells in response to Wort, or Wort-loaded MSC-CM. Phosphorylated forms of Akt and mTOR proteins were assessed in cells treated with different concentrations of Wort (7A), or Wort-loaded CM (7B) progressively reduced relative to the untreated cells and the corresponding β-actin, as a loading control. Both “7C” and “7D” depict the corresponding band intensities. Expression of P-AKT, m-mTOR at the mRNA level is shown in “7E”, whereas “7F” demonstrates the stepwise downregulation of PI3K-catalytic subunit (PI3K-Ca)