Fig. 1
Representative images of plants inoculated with CMV-Y and of RT-PCR analyses to detect CMV-Y infections. (a-b) Plants from NIL-M and line 03-336 at 8 days post-inoculation (dpi). (c) Plants from NIL-M, line 03-336, and line 03–009 at 15 dpi. (d) RT-PCR analysis of CMV-Y infections in NIL-M and line 03-336. (e) F1 plants from line 03-336 and NIL-M at 11 dpi. Arrows indicate physical damage caused by rub-inoculation. (f) RT-PCR analysis of CMV-Y infections in F1 plants. (g) F2 progeny derived from plants shown in (e) at 14 dpi. (h) RT-PCR analysis of CMV-Y infections in F2 progeny derived from plants shown in (e). GAPDH was amplified as a positive control in RT-PCR analyses (d, f, h). R: resistant (asymptomatic); S: susceptible (symptomatic); M: mock-inoculation control; I: inoculated