Table 1 Reagents and equipment used for antibody screening and identification. The CAT card, volume of RRBC, volume of plasma, heating block and centrifuge used in testing were in accordance with the instructions for use (IFUs) published by the various RRBC manufacturers
CAT card | Screening cells | Panel Cells | Volume of RRBC (µL) | Volume of plasma (µL) | Heating block | Centrifuge | |
|---|---|---|---|---|---|---|---|
Test Script 1 | Bio-Rad ID-Card LISS/Coombs card | Immulab 0.8% Abtectcell III + Bio-Rad 0.8% ID-DiaCell I-II-III | Immulab 0.8% Phenocell Panel B + Bio-Rad 0.8% ID-DiaPanel | 50 | 25 | Grifols DG Therm heating block | Bio-Rad ID-Centrifuge 12 S II centrifuge |
Test Script 2 | Grifols DG Gel Coombs card | Immulab 0.8% Abtectcell III + Grifols 0.8% Perfect Screen 3 | Immulab 0.8% Phenocell Panel B + Grifols 0.8% Identisera Diana | 50 | 25 | Grifols DG Therm heating block | Grifols DG Spin centrifuge |
Test Script 3 | Ortho BioVue Anti-IgG, -C3d polyspecific CAT card | Immulab 0.8% Abtectcell III + Ortho 0.8% Surgiscreen | Immulab 0.8% Phenocell Panel B + Ortho 0.8% Panel A | 50 | 40 | Ortho BioVue System heating block | Ortho BioVue System centrifuge |