The context of blaOXA−23 gene in Iraqi carbapenem-resistant Acinetobacter baumannii isolates belonging to global clone 1 and global clone 2

Table 1 Primers used for PCR assays

PCR	Primer	Sequence (5ʹ-3ʹ)*	Annealing temperature (˚ C)	Amplicon length (bp)	Reference
Group1	ompAF306 ompAR660 csuEF csuER oxa66F89 oxa66R647	F: GATGGCGTAAATCGTGGTA R: CAACTTTAGCGATTTCTGG F: CTTTAGCAAACATGACCTACC R: TACACCCGGGTTAATTCGT F: GCGCTTCAAAATCTGATGTA R: GCGTATATTTTGTTTCCATTC	57	355 702 559	[23]
Group2	ompAF378 ompAR660 csuEF csuER oxa69F169 oxa69R330	F: GACCTTTCTTATCACAACGA R: CAACTTTAGCGATTTCTGG F: GGCGAACATGATCTATTT R: CTTCATGGCTCGTTGGTT F: CATCAAGGTCAAACTCAA R: TAGCCTTTTTTCCCATC	57	343 580 162	[23]
bla_OXA−23	oxa23F-like oxa23R-like	F: GATCGGATTGGAGAACCAGA R: ATTTCTGACCGCATTTCCAT	60	501	[20]
bla_OXA−24	oxa24F-like oxa24R-like	F: GGTTAGTTGGCCCCTTAAA R: AGTTGAGCGAAAAGGGGATT	60	246
bla_OXA−58	oxa58F-like oxa58R-like	F: AAGTATTGGGGCTTGTGCTG R: CCCCTCTGCGCTCTACATAC	60	599
bla_OXA−Ab	OXA-69 A OXA-69B	F: CTAATAATTGATCTACTCAAG R: CCAGTGGATGGATGGATAGATTATC	57	975	[24]
ISAba1-oxa23	ISAba1B oxa23-F	R: CATGTAAACCAATCGTCACC F: GATCGGATTGGAGAACCAGA	59	2725	[17] [25]
oxa23- ISAba1	oxa23-R ISAba1B	R: ATTTCTGACCGCATTTCCAT F: CATGTAAACCAATGCTCACC	59	1369	[25] [17]

ISSN: 1756-0500