Fig. 3
Long term storage and freeze–thaw cycles do not alter lncRNA or mRNA expression. qRT-PCR gene expression data were produced for a six lncRNAs and b six mRNAs. Baseline RNA immediately isolated from PAXgene Blood RNA tubes was compared to three treatment conditions including RNA storage at − 80 °C for one year, total RNA from PAXgene tubes exposed to five freeze–thaw cycles, and total RNA that was exposed to ten freeze–thaw cycles. Paired sample t-test was used to determine statistical significance. Bars represent mean Log2(ΔΔCt) with SD for n = 5 individual healthy subject’s baseline total RNA samples and after one of the three treatment conditions