Figure 1
From: Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design
Relative location of siRNA target sites and primer amplicons. (A) Target mRNA sequence of each PKCε siRNA duplex, and sequences for each PKCε primer set. (B) Location of primer amplicons (set 1-5) in relation to the sites of siRNA directed cleavage (siRNA 1 and siRNA 2) of human PKCε mRNA.