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Table 2 Sequence specific oligonucleotide used for sequencing of HLA-A*02, B*46 and DRB

From: Identification of HLA-A*02-B*46 haplotype allele variant in Guangdong Han populations on the basis of PCR-SBT

Gene Names

Primer Names

Nucleotide sequence(5'-3')

Tm°

Position

HLA-A Exon 2

A5.9 2F

5'-TCG GGC (A/G)GG TCT CAG CC-3'

60

4934–4950a

 

AEx2 2R

5'-CAC TCA CCG GCC TCG CTC TGG-3'

68

5222–5242a

HLA-A Exon 3

A5.10 3F

5'-GGG CTC GGG GGA C(C/T)G GG-3'

64

5438–5454a

 

A3.4 3R

5'-GAG GCG CCC CGT GGC-3'

58

5762–5776a

HLA-B Exon 2

BEX 2F

5'-GGG CGC AGG ACC (T/C)G(A/G) GGA-3'

58

5602–5619b

 

BEX 2R

5'-GGT CAC TCA CCG (G/T)CC TCG-3'

58

5272–5289b

HLA-B Exon 3

BEX 3F

5'-GGG GCC AGG GTC TCA CA-3'

58

5028–5044b

 

BEX 3R

5'-CCC ACT GCC CCT GGT ACC-3'

62

4744–4761b

HLA-DRB1 Exon 2

21M13F

5'-TGT AAA ACG ACG GCC AGT-3'

54

-,*

 

M13R

5'-CAG GAA AGA GCT ATG ACC-3'

54

-,*

  1. a, Numbering of the HLA-A sequence is from the GeneBank accession NT_007952
  2. b, Numbering of the HLA-B sequence is from the GeneBank accession NT_007592
  3. *, Numbering of the HLA-DRB1 sequence is from the reference 7 and http://www.ihwg.org